#Puc19 snapgene full#
#Puc19 snapgene software#
#Puc19 snapgene series#

Web puc19 sequences (3) addgene sequences: Coli plasmid cloning vector containing portions of pbr322 and m13mp19. coli with pBB275 than pUC19 (Fig 2D and E). This is consistent with the observed larger fraction of the GFP ON cells for SOS reporter in E. coli MG1655 harboring pUC19 in monoculture and in the co-culture with engineered B. Molecular cloning is a set of experimental methods in molecular biology that are used to. Created with SnapGene Pspfl (26801 (2655) BseYI 125501 AlwNI BR322ori-F (118137) Afill - Peil (273) 25001 1095 CAP binding site Mre MCS tac promoter pUC19 2686 bp L4440 0711881 Espoi - Sapl 4390I M13/UC Reverse (584.606) M13 Reverse 1603.6191 Hindill (632) BuAl - BspMI (637) Sphl (642) Pstl - Sbf 1548) Sall 1650) Acel 00511 Hincil 1652) Xbal (6561 BamHI (662) Aval. GSL Biotech SnapGene 2021 is a reliable and powerful application that allows molecular biologists to create, browse, and share richly annotated DNA sequence files. thailandensis.Here is an image of a plasmid (pUC19), which one is the light green gene marked with '?' Note: You are planning to SELECT the transformed colonies in media containing the antibiotic ampicillin. Validation of these improved suicide vectors was demonstrated by the genetic inactivation of selected genes in the Bcc species Burkholderia cenocepacia and B.

#Puc19 snapgene software#

Use with SnapGene software or the free Viewer to visualize additional data and align other sequences.

This implies that sequences that differentiate pUC19 and pBB275 (e.g., f1 ori and ycgO) could contribute to plasmid multimerization. SnapGene File: Plasmid sequence and SnapGene enhanced annotations.

#Puc19 snapgene series#

These were then used in combination with and/or to construct a series enhanced suicide vectors, pSHAFT2, pSHAFT3 and pSHAFT-GFP to facilitate effective allelic replacement in the Bcc. The plasmid conformations of pUC19 derived from recA+ E. Here we describe the construction of improved antibiotic-resistance cassettes that specify resistance to kanamycin, chloramphenicol or trimethoprim effectively in the Bcc and related species. In the last decade, the cultivated area of maize has increased in Central Europe due to its high yield potential and diverse uses for feed and bio-energy. However, due to their intrinsic resistance to many antibiotics only selected antibiotic-resistance markers can be used in members of the genus Burkholderia, including the Burkholderia cepacia complex (Bcc).

#Puc19 snapgene full#

Allelic replacement is often carried out by disruption of the gene of interest by insertion of an antibiotic-resistance marker followed by subsequent transfer of the mutant allele to the genome of the host organism in place of the wild-type gene. pUC19 Sequences (3) Addgene Sequences: Full (1) Partial (2) Full Sequences from Addgene (1) Based on next-generation sequencing (NGS) results where indicated (Addgene NGS Result), or assembled from reference sequences and/or Sanger results (Addgene Assembled Sequence). SnapGene SnapGene is the easiest way to plan, visualize and document your.

To elucidate the function of a gene in bacteria it is vital that targeted gene inactivation (allelic replacement) can be achieved. coli vector with a multiple cloning site (MCS) for DNA cloning.